oSIST prEN ISO 25202:2025
(Main)Leather - Chemical analysis - Determination of glutaraldehyde content (ISO/DIS 25202:2024)
Leather - Chemical analysis - Determination of glutaraldehyde content (ISO/DIS 25202:2024)
This international standard specifies a method for the determination of the content of Leather.
This method is based on high performance liquid chromatography (HPLC). It is selective and not sensitive to coloured extracts.
Leder - Chemische Analyse - Bestimmung des Glutaraldehydgehalts (ISO/DIS 25202:2024)
Cuir - Analyse chimique - Détermination de la teneur en glutaraldéhyde (ISO/DIS 25202:2024)
Usnje - Kemijska analiza - Določevanje vsebnosti glutaraldehida (ISO/DIS 25202:2024)
General Information
- Status
- Not Published
- Public Enquiry End Date
- 27-Feb-2025
- Technical Committee
- IUSN - Leather
- Current Stage
- 4020 - Public enquire (PE) (Adopted Project)
- Start Date
- 19-Dec-2024
- Due Date
- 08-May-2025
- Completion Date
- 03-Mar-2025
Overview
oSIST prEN ISO 25202:2025 is an international standard developed by the Slovenian Institute for Standardization (SIST) in alignment with ISO and CEN requirements. This standard, titled "Leather - Chemical analysis - Determination of glutaraldehyde content," details a precise laboratory method for measuring free and released glutaraldehyde in leather using high-performance liquid chromatography (HPLC or LC). The protocol is specifically designed to be selective and robust, even when colored extracts are present, ensuring reliable results critical to the leather and tanning industries.
Key Topics
- Glutaraldehyde Determination: The standard focuses on the accurate detection and quantification of glutaraldehyde, a key chemical used in tanning processes to impart unique properties to leather.
- High-Performance Liquid Chromatography (HPLC/LC): The method involves extraction, derivatization using 2,4-dinitrophenylhydrazine (DNPH), and subsequent quantification using reversed-phase LC with ultraviolet (UV) detection.
- Sample Preparation: Offers guidance on selecting and preparing leather specimens in accordance with related ISO standards to ensure consistency and repeatability.
- Data Reporting: Specifies clear directives for expressing test results in mg/kg, calibration procedures, and the critical information required in laboratory reports.
Applications
The practical applications of oSIST prEN ISO 25202:2025 are broad and relevant to:
- Tanning Industry: Ensures compliance with chemical safety standards in leather manufacturing by quantifying residual glutaraldehyde, thereby supporting product safety and quality assurance.
- Regulatory Compliance: Facilitates conformity with national and international regulations concerning chemical substances in leathers, enabling traceability and transparency in supply chains.
- Product Testing and Certification: Supports laboratories in certifying leather and finished goods (shoes, garments, upholstery) for the presence of glutaraldehyde, ensuring products meet both customer expectations and legal requirements.
- Research and Development: Assists R&D teams in optimizing tanning formulas and evaluating new processes by providing reliable, standardized chemical analysis tools.
Related Standards
For comprehensive chemical and physical analysis of leather, the following standards are closely linked with oSIST prEN ISO 25202:2025:
- ISO 2418: Leather - Chemical, physical, mechanical and fastness tests - Position and preparation of specimens for testing.
- ISO 4044: Leather - Chemical tests - Preparation of chemical test samples.
- ISO 3696: Water for analytical laboratory use - Specification and test methods.
- ISO 17226-1: Leather - Chemical determination of formaldehyde content - Part 1: Method using high-performance liquid chromatography.
- IULTCS/IUC 40: International methods for sampling and testing of leather, serving as the technical origin for this analysis method.
Practical Value
Implementing oSIST prEN ISO 25202:2025 offers distinct advantages:
- Consistency in Testing: Ensures that all laboratories and manufacturers follow a standardized procedure, resulting in comparable and credible test results.
- Market Confidence: Provides proof of compliance for buyers, regulators, and end-users, boosting confidence in the safety, quality, and traceability of leather goods.
- Risk Management: Identifies and quantifies potentially hazardous residues, helping stakeholders manage chemical risks and meet increasingly stringent international environmental and safety regulations.
This standard is essential for laboratories, manufacturers, compliance professionals, and regulators involved in the production and certification of leather products, supporting both quality control and product stewardship.
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Frequently Asked Questions
oSIST prEN ISO 25202:2025 is a draft published by the Slovenian Institute for Standardization (SIST). Its full title is "Leather - Chemical analysis - Determination of glutaraldehyde content (ISO/DIS 25202:2024)". This standard covers: This international standard specifies a method for the determination of the content of Leather. This method is based on high performance liquid chromatography (HPLC). It is selective and not sensitive to coloured extracts.
This international standard specifies a method for the determination of the content of Leather. This method is based on high performance liquid chromatography (HPLC). It is selective and not sensitive to coloured extracts.
oSIST prEN ISO 25202:2025 is classified under the following ICS (International Classification for Standards) categories: 59.140.30 - Leather and furs; 71.040.40 - Chemical analysis. The ICS classification helps identify the subject area and facilitates finding related standards.
oSIST prEN ISO 25202:2025 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Usnje - Kemijska analiza - Določevanje vsebnosti glutaraldehida (ISO/DIS
25202:2024)
Leather - Chemical analysis - Determination of glutaraldehyde content (ISO/DIS
25202:2024)
Leder - Chemische Analyse - Bestimmung des Glutaraldehydgehalts (ISO/DIS
25202:2024)
Cuir - Analyse chimique - Détermination de la teneur en glutaraldéhyde (ISO/DIS
25202:2024)
Ta slovenski standard je istoveten z: prEN ISO 25202
ICS:
59.140.30 Usnje in krzno Leather and furs
71.040.40 Kemijska analiza Chemical analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
DRAFT
International
Standard
ISO/DIS 25202
IULTCS
IUC 40
IULTCS
Leather — Chemical analysis —
Secretariat: ISO
Determination of glutaraldehyde
content
Voting begins on:
2024-12-11
ICS: 59.140.30
Voting terminates on:
2025-03-05
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS.
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
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Reference numbers
ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
DRAFT
ISO/DIS 25202:2024(en)
International
Standard
ISO/DIS 25202
IULTCS
IUC 40
IULTCS
Leather — Chemical analysis —
Secretariat: ISO
Determination of glutaraldehyde
content
Voting begins on:
ICS: 59.140.30
Voting terminates on:
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2024
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
BE CONSIDERED IN THE LIGHT OF THEIR
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
or ISO’s member body in the country of the requester.
NATIONAL REGULATIONS.
ISO copyright office
RECIPIENTS OF THIS DRAFT ARE INVITED
CP 401 • Ch. de Blandonnet 8
TO SUBMIT, WITH THEIR COMMENTS,
CH-1214 Vernier, Geneva
NOTIFICATION OF ANY RELEVANT PATENT
Phone: +41 22 749 01 11
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Email: copyright@iso.org
Website: www.iso.org
Reference numbers
Published in Switzerland ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
ii
ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Preparation of test specimens . 2
8 Procedure . 3
8.1 Sample processing .3
8.1.1 Extraction .3
8.1.2 Reaction with DNPH .3
8.2 Determination by LC .3
9 Expression of results . 4
9.1 General .4
9.2 Calculation of the glutaraldehyde content .4
9.3 Precision of the test method .4
10 Test report . 4
Annex A (informative) LC conditions . 5
Bibliography . 6
iii
ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
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with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO [had/had not] received notice of
(a) patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
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This document was prepared by the European Committee for Standardization (CEN) Technical Committee
CEN/TC 289, Leather, in collaboration with the Chemical Test Commission of the International Union of
Leather Technologists and Chemists Societies (IUC Commission, IULTCS), in accordance with the Agreement
on technical cooperation between ISO and CEN (Vienna Agreement). This method is technically similar to
the Colorimetric Section of the method IUC 19 which was declared an official method at the IULTCS Delegates
meeting on 31st May 2003 i
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