oSIST prEN ISO 10519:2026

SLOVENSKI STANDARD
01-julij-2026
Seme oljne repice - Določevanje klorofila - Spektrometrijska metoda (ISO/FDIS
10519:2026)
Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO/FDIS
10519:2026)
Rapssamen - Bestimmung des Chlorophyllgehaltes - Spektrometrisches Verfahren
(ISO/FDIS 10519:2026)
Graines de colza - Détermination de la teneur en chlorophylle - Méthode
spectrométrique (ISO/FDIS 10519:2026)
Ta slovenski standard je istoveten z: prEN ISO 10519
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

FINAL DRAFT
International
Standard
ISO/FDIS 10519
ISO/TC 34/SC 2
Rapeseed — Determination
Secretariat: AFNOR
of chlorophyll content —
Voting begins on:
Spectrometric method
2026-06-03
Graines de colza — Détermination de la teneur en chlorophylle —
Voting terminates on:
Méthode spectrométrique
2026-08-26
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/CEN PARALLEL PROCESSING LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 10519:2026(en) © ISO 2026

FINAL DRAFT
ISO/FDIS 10519:2026(en)
International
Standard
ISO/FDIS 10519
ISO/TC 34/SC 2
Rapeseed — Determination
Secretariat: AFNOR
of chlorophyll content —
Voting begins on:
Spectrometric method
Graines de colza — Détermination de la teneur en chlorophylle —
Voting terminates on:
Méthode spectrométrique
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2026
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ISO/FDIS 10519:2026(en) © ISO 2026

ii
ISO/FDIS 10519:2026(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagent . 1
6 Apparatus . 2
7 Sampling . 2
8 Preparation of test sample . 3
9 Procedure . 3
9.1 Test portions .3
9.2 Extraction .3
9.3 Determination .3
10 Expression of results . 4
11 Precision . 4
11.1 Repeatability .4
11.2 Reproducibility .4
12 Test report . 4
Annex A (informative) Results of interlaboratory test . 6
Annex B (informative) Preparation of the calibration curve to determine the k factor . 7
Bibliography . 9

iii
ISO/FDIS 10519:2026(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 2, Oleaginous seeds and fruits and oilseed meals, in collaboration with the European Committee for
Standardization (CEN) Technical Committee CEN/TC 307, Oilseeds, vegetable and animal fats and oils and their
by-products - Methods of sampling and analysis, in accordance with the Agreement on technical cooperation
between ISO and CEN (Vienna Agreement).
This fourth edition cancels and replaces the third edition (ISO 10519:2015), of which it constitutes a minor
revision. It also incorporates the Amendment ISO 10519:2015/Amd 1:2025.
The changes are as follows:
— Annex B “Preparation of the calibration curve to determine the k factor” has been added.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
FINAL DRAFT International Standard ISO/FDIS 10519:2026(en)
Rapeseed — Determination of chlorophyll content —
Spectrometric method
1 Scope
This document specifies a spectrometric method for the determination of the chlorophyll content of
rapeseed. It is not applicable to the determination of chlorophyll in oils.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 648, Laboratory glassware — Single-volume pipettes
ISO 664, Oilseeds — Reduction of laboratory sample to test sample
ISO 665, Oilseeds — Determination of moisture and volatile matter content
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
chlorophyll content
mass fraction of substances in the sample contributing to the absorption band at a wavelength near 665 nm,
measured as chlorophyll A
Note 1 to entry: The chlorophyll conten
...