SIST EN ISO 9308-1:2014/A1:2017
(Amendment)Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016)
Wasserbeschaffenheit Zählung von Escherichia coli und coliformen Bakterien Teil 1: Membranfiltrationsverfahren für Wässer mit niedriger Begleitflora (ISO 9308-1:2014/Amd 1:2016)
Qualité de l'eau - Dénombrement des Escherichia coli et des bactéries coliformes - Partie 1: Méthode par filtration sur membrane pour les eaux à faible teneur en bactéries (ISO 9308-1:2014/Amd 1:2016)
Kakovost vode - Ugotavljanje števila Escherichia coli in koliformnih bakterij - 1. del: Metoda membranske filtracije za vode z majhnim številom spremljajočih bakterij - Dopolnilo A1 (ISO 9308-1:2014/Amd 1:2016)
General Information
- Status
- Published
- Public Enquiry End Date
- 05-Apr-2016
- Publication Date
- 16-May-2017
- Technical Committee
- KAV - Water quality
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 11-Apr-2017
- Due Date
- 16-Jun-2017
- Completion Date
- 17-May-2017
- Directive
- Slovenian Regulation2008-01-0974 - Uredba o upravljanju kakovosti kopalnih voda
Slovenian Regulation2014-01-3849 - Pravilnik o prvih meritvah in obratovalnem monitoringu odpadnih voda
Slovenian Regulation2015-01-2455 - Pravilnik o minimalnih higienskih zahtevah, ki jih morajo izpolnjevati kopališča in kopalna voda v bazenih
Slovenian RegulationTP262 - Uredba o pitni vodi
Harmonized StandardTRRTP178 - Uredba o upravljanju kakovosti kopalnih voda
Relations
- Effective Date
- 09-Feb-2026
- Effective Date
- 28-Jan-2026
- Effective Date
- 28-Jan-2026
- Effective Date
- 01-Mar-2016
Overview
SIST EN ISO 9308-1:2014/A1:2017 specifies the standardized membrane filtration method for the enumeration of Escherichia coli and coliform bacteria in waters with low bacterial background flora. As an amendment to ISO 9308-1:2014, it aligns with EU regulations and ensures reliable water quality testing crucial for public health, environmental monitoring, and compliance with regulatory requirements.
The standard is overseen by the Slovenian Institute for Standardization (SIST) and supports consistent microbiological analysis procedures across Europe, facilitating both national and international water quality management.
Key Topics
- Microbiological Analysis of Water: Focuses on detecting and counting E. coli and coliform bacteria using membrane filtration techniques, which are essential indicators of water safety.
- Membrane Filtration Method: Provides procedures for filtering water samples to efficiently capture bacteria present in low concentrations, followed by incubation for optimal bacterial colony development.
- Incubation Specifications: The updated method specifies incubation of membrane filters at 36 ± 2°C for 21 to 24 hours for accurate results.
- Chromogenic Coliform Agar (CCA): Use of CCA medium for differential detection, enabling the identification of E. coli (dark-blue to violet colonies) and other coliforms (pink to red colonies) by color change after incubation.
- Performance Testing: Emphasizes criteria such as productivity, selectivity, and specificity in quality control of the testing media, ensuring the reliability and repeatability of results.
Applications
- Drinking Water Monitoring: Essential for ensuring safe potable water supplies, meeting regulations for microbial contaminants, and maintaining public health.
- Environmental Water Testing: Used to assess the microbiological quality of rivers, lakes, reservoirs, and recreational waters where low background flora require sensitive detection methods.
- Process and Bottled Water Control: Applied in industries that rely on high-purity water, including pharmaceuticals, food and beverage production, and other sectors requiring stringent microbiological standards.
- Regulatory Compliance: Assists water utilities, laboratories, and regulatory bodies in adhering to national and European directives on water quality by providing a harmonized and validated testing protocol.
- Research and Development: Supports scientific studies on microbial water pollution, pollution source tracking, and waterborne disease risk assessment.
Related Standards
- ISO 9308-1:2014: The original international standard detailing the enumeration of E. coli and coliform bacteria in waters using membrane filtration.
- EN ISO 9308-1:2014: The European adoption of the ISO standard ensuring harmonization across EU member states.
- ISO 7899 (Water quality - Detection and enumeration of intestinal enterococci): Related standard for broader microbiological water quality analysis.
- ISO 19458 (Sampling for microbiological analysis): Provides guidance on sampling strategies and sample handling to support accuracy in microbiological water testing.
Practical Value
Implementing SIST EN ISO 9308-1:2014/A1:2017 offers significant practical benefits, including:
- Reliable Detection: Provides a validated framework for sensitive and specific detection of microbial indicators, supporting effective water safety management.
- Standards Compliance: Ensures alignment with European and international best practices, facilitating regulatory compliance and market access.
- Public Health Protection: Aids in early identification of fecal contamination in water sources, reducing the risk of waterborne disease outbreaks and supporting community health.
- Quality Assurance: The standardized method enhances repeatability and comparability of results between laboratories, aiding in environmental monitoring programs and inter-laboratory studies.
By integrating this standard into water quality monitoring protocols, organizations can ensure high levels of precision and confidence in the microbiological analysis of waters with low background flora. This, in turn, underpins safe water supply, regulatory adherence, and environmental protection.
Get Certified
Connect with accredited certification bodies for this standard
CIS Institut d.o.o.
Personal Protective Equipment (PPE) certification body. Notified Body NB-2890 for EU Regulation 2016/425 PPE.
Kiwa BDA Testing
Building and construction product certification.
Kmetijski inštitut Slovenije
Agricultural Institute of Slovenia. Soil testing, plant health, agricultural product analysis.
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Frequently Asked Questions
SIST EN ISO 9308-1:2014/A1:2017 is a amendment published by the Slovenian Institute for Standardization (SIST). Its full title is "Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016)". This standard covers: Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016)
Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016)
SIST EN ISO 9308-1:2014/A1:2017 is classified under the following ICS (International Classification for Standards) categories: 07.100.20 - Microbiology of water; 13.060.70 - Examination of biological properties of water. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 9308-1:2014/A1:2017 has the following relationships with other standards: It is inter standard links to SIST ISO 3696:1995, SIST EN 15382:2013, SIST EN 71-20:2026, SIST EN ISO 9308-1:2014. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
SIST EN ISO 9308-1:2014/A1:2017 is associated with the following European legislation: EU Directives/Regulations: 2008-01-0974, 2014-01-3849, 2015-01-2455, TP262, TRRTP178. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.
SIST EN ISO 9308-1:2014/A1:2017 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
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Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane
filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd
1:2016)
Wasserbeschaffenheit Zählung von Escherichia coli und coliformen Bakterien Teil 1:
Membranfiltrationsverfahren für Wässer mit niedriger Begleitflora (ISO 9308-1:2014/Amd
1:2016)
Qualité de l'eau - Dénombrement des Escherichia coli et des bactéries coliformes -
Partie 1: Méthode par filtration sur membrane pour les eaux à faible teneur en bactéries
(ISO 9308-1:2014/Amd 1:2016)
Ta slovenski standard je istoveten z: EN ISO 9308-1:2014/A1:2017
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 9308-1:2014/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2017
EUROPÄISCHE NORM
ICS 07.100.20
English Version
Water quality - Enumeration of Escherichia coli and
coliform bacteria - Part 1: Membrane filtration method for
waters with low bacterial background flora (ISO 9308-
1:2014/Amd 1:2016)
Qualité de l'eau - Dénombrement des Escherichia coli Wasserbeschaffenheit Zählung von Escherichia coli
et des bactéries coliformes - Partie 1: Méthode par und coliformen Bakterien Teil 1:
filtration sur membrane pour les eaux à faible teneur Membranfiltrationsverfahren für Wässer mit niedriger
en bactéries (ISO 9308-1:2014/Amd 1:2016) Begleitflora (ISO 9308-1:2014/Amd 1:2016)
This amendment A1 modifies the European Standard EN ISO 9308-1:2014; it was approved by CEN on 18 January 2017.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 9308-1:2014/A1:2017 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 9308-1:2014/A1:2017) has been prepared by Technical Committee ISO/TC 147
“Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat
of which is held by DIN.
This Amendment to the European Standard EN ISO 9308-1:2014 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by July 2017, and
conflicting national standards shall be withdrawn at the latest by July 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 9308-1:2014/Amd 1:2016 has been approved by CEN as EN ISO 9308-1:2
...