EN ISO 13365-2:2020
(Main)Leather - Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography - Part 2: Artificial perspiration extraction method (ISO 13365-2:2020)
General Information
- Abstract
This document specifies a test method by artificial perspiration solution aqueous extraction for the determination of the aqueous extractable content of the following preservative agents in leather by liquid chromatography:
— 2-(thiocyanomethylthio)-benzothiazole (TCMTB);
— 4-chloro-3-methylphenol (PCMC);
— 2-phenylphenol (OPP);
— 2-octylisothiazol-3(2H)-one (OIT);
This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.
- Status
- Published
- Publication Date
- 01-Sep-2020
- Withdrawal Date
- 30-Mar-2021
- Technical Committee
- CEN/TC 289 - Leather
- Drafting Committee
- CEN/TC 289/WG 1 - Chemical test methods
- Current Stage
- 6060 - Definitive text made available (DAV) - Publishing
- Start Date
- 02-Sep-2020
- Completion Date
- 02-Sep-2020
Overview
EN ISO 13365-2:2020 - “Leather - Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography - Part 2: Artificial perspiration extraction method” - is a CEN-adopted ISO test method for measuring the aqueous extractable content of common leather preservatives. The method uses an artificial perspiration (acid) aqueous extraction followed by analysis by liquid chromatography (HPLC) with UV or MS detection. It is intended to assess potential user exposure to preservative agents released from leather during use.
Key Topics and Requirements
- Target compounds: 2-(thiocyanomethylthio)-benzothiazole (TCMTB), 4-chloro-3-methylphenol (PCMC), 2-phenylphenol (OPP), 2-octylisothiazol-3(2H)-one (OIT); method can also detect their breakdown products.
- Extraction principle: aqueous extraction with an acid artificial perspiration solution in a thermostatic flask shaker; filtered extract is analysed by HPLC (UV or MS).
- Reagents and purity: reference standards (≥97–99.7 % for target compounds), HPLC/LC‑MS grade solvents, deionized water (ISO 3696 grade 3).
- Calibration: at least four calibration solutions covering a wide concentration range (examples in the standard include 2, 10, 25, 50 and 100 mg/L); calibration solutions stored at (4 ± 3) °C.
- Apparatus: HPLC system with UV or MS detector; reversed-phase C8 or C18 column (with pre-column); water bath maintaining (37 ± 2) °C with horizontal/orbital shaker (specified speeds); membrane filter (polyamide, 0.45 µm); analytical balance (0.1 mg).
- Sampling and sample prep: sampling per ISO 2418 and sample preparation per ISO 4044 (with guidance for finished products and wet intermediates).
- Chromatographic conditions: included in Annex A of the standard, with additional conditions for MS detection.
Applications and Who Uses It
- Quality control laboratories in tanneries and finished leather manufacturers to quantify preservative residues.
- Independent testing labs performing regulatory or product-safety testing for footwear, garments, furniture and other leather goods.
- Compliance and chemical-safety teams assessing consumer exposure risk and ensuring conformity with chemical regulations or internal safety criteria.
- R&D and formulation groups evaluating preservative performance and degradation (breakdown products) under simulated use conditions.
- Practical uses include evaluating leathers for microbiological protection efficacy, monitoring extractable preservative release under simulated perspiration, and supporting material safety data.
Related Standards
- ISO 13365 series (this is Part 2; Part 1 complements the series)
- ISO 2418 (sampling locations), ISO 4044 (sample preparation), ISO 3696 (water for analytical use), ISO 4684 (volatile matter)
Keywords: EN ISO 13365-2:2020, leather preservative determination, TCMTB, PCMC, OPP, OIT, artificial perspiration extraction, HPLC, LC‑MS, aqueous extraction, leather testing.
Relations
- Effective Date
- 08-Jun-2022
- Effective Date
- 25-Aug-2026
- Refers
ISO 2418:2017 - Leather — Chemical, physical and mechanical and fastness tests — Sampling location - Effective Date
- 09-Feb-2026
- Effective Date
- 09-Feb-2026
- Effective Date
- 09-Feb-2026
- Effective Date
- 28-Jan-2026
- Effective Date
- 12-Feb-2026
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Frequently Asked Questions
EN ISO 13365-2:2020 is a standard published by the European Committee for Standardization (CEN). Its full title is "Leather - Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography - Part 2: Artificial perspiration extraction method (ISO 13365-2:2020)". This standard covers: This document specifies a test method by artificial perspiration solution aqueous extraction for the determination of the aqueous extractable content of the following preservative agents in leather by liquid chromatography: — 2-(thiocyanomethylthio)-benzothiazole (TCMTB); — 4-chloro-3-methylphenol (PCMC); — 2-phenylphenol (OPP); — 2-octylisothiazol-3(2H)-one (OIT); This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.
This document specifies a test method by artificial perspiration solution aqueous extraction for the determination of the aqueous extractable content of the following preservative agents in leather by liquid chromatography: — 2-(thiocyanomethylthio)-benzothiazole (TCMTB); — 4-chloro-3-methylphenol (PCMC); — 2-phenylphenol (OPP); — 2-octylisothiazol-3(2H)-one (OIT); This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.
EN ISO 13365-2:2020 is classified under the following ICS (International Classification for Standards) categories: 59.140.30 - Leather and furs. The ICS classification helps identify the subject area and facilitates finding related standards.
EN ISO 13365-2:2020 has the following relationships with other standards: It is inter standard links to EN ISO 13365:2011, ISO 4684:2005, ISO 2418:2017, ISO 4044:2017, ISO 3696:1987, EN 1457-1:2012, ISO 13365-2:2020. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
EN ISO 13365-2:2020 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
01-oktober-2020
Nadomešča:
SIST EN ISO 13365:2011
Usnje - Kemijsko določevanje sredstev za zaščito (TCMTB, PCMC, OPP, OIT) v
usnju s tekočinsko kromatografijo - 2. del: Metoda ekstrakcije umetnega znojenja
(ISO 13365-2:2020)
Leather - Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT)
content in leather by liquid chromatography - Part 2: Artificial perspiration extraction
method (ISO 13365-2:2020)
Leder - Chemische Prüfungen - Bestimmung von Konservierungsmitteln (TCMTB, CMK,
OPP, OIT) in Leder mittels Flüssigchromatographie - Teil 2: Extrahierbarer Anteil (ISO
13365-2:2020)
Cuir - Dosage chimique des agents de conservation (TCMTB, PCMC, OPP, OIT) dans le
cuir par chromatographie en phase liquide - Partie 2: Extraction à la sueur artificielle
(ISO 13365-2:2020)
Ta slovenski standard je istoveten z: EN ISO 13365-2:2020
ICS:
59.140.30 Usnje in krzno Leather and furs
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 13365-2
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2020
EUROPÄISCHE NORM
ICS 59.140.30 Supersedes EN ISO 13365:2011
English Version
Leather - Chemical determination of the preservative
(TCMTB, PCMC, OPP, OIT) content in leather by liquid
chromatography - Part 2: Artificial perspiration extraction
method (ISO 13365-2:2020)
Cuir - Dosage chimique des agents de conservation Leder - Chemische Prüfungen - Bestimmung von
(TCMTB, PCMC, OPP, OIT) dans le cuir par Konservierungsmitteln (TCMTB, CMK, OPP, OIT) in
chromatographie en phase liquide - Partie 2: Leder mittels Flüssigchromatographie - Teil 2:
Extraction à la sueur artificielle (ISO 13365-2:2020) Extrahierbarer Anteil (ISO 13365-2:2020)
This European Standard was approved by CEN on 2 July 2020.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 13365-2:2020 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 13365-2:2020) has been prepared by Technical Committee ISO/IULTCS
"International Union of Leather Technologists and Chemists Societies" in collaboration with Technical
Committee CEN/TC 289 “Leather” the secretariat of which is held by UNI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2021, and conflicting national standards shall
be withdrawn at the latest by March 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 13365:2011.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 13365-2:2020 has been approved by CEN as EN ISO 13365-2:2020 without any
modification.
INTERNATIONAL ISO
STANDARD 13365-2
IULTCS
IUC 29-2
First edition
2020-07
Leather — Chemical determination
of the preservative (TCMTB, PCMC,
OPP, OIT) content in leather by liquid
chromatography —
Part 2:
Artificial perspiration extraction
method
Cuir — Dosage chimique des agents de conservation (TCMTB, PCMC,
OPP, OIT) dans le cuir par chromatographie en phase liquide —
Partie 2: Extraction à la sueur artificielle
Reference numbers
ISO 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
©
ISO 2020
ISO 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
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Email: copyright@iso.org
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Published in Switzerland
ii © ISO 2020 – All rights reserved
ISO 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
5.1 General . 2
5.2 Target compounds . 2
5.3 Preparation of calibration solutions . 2
6 Apparatus and materials. 3
7 Testing procedure . 3
7.1 Sampling and sample preparation . 3
7.2 Preparation of acid artificial perspiration solution . 3
7.3 Preparation of analytical solution . 3
7.4 Analytical procedure . 4
8 Calculation . 4
9 Test report . 5
Annex A (informative) Chromatographic conditions . 6
ISO 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
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