EN ISO 18219-1:2021

SLOVENSKI STANDARD
01-september-2021
Nadomešča:
SIST EN ISO 18219:2015
Usnje - Določevanje kloriranih ogljikovodikov v usnju - 1. del: Kromatografska
metoda za kratke verige kloriranih parafinov (SCCP) (ISO 18219-1:2021)
Leather - Determination of chlorinated hydrocarbons in leather - Part 1: Chromatographic
method for short-chain chlorinated paraffins (SCCPs) (ISO 18219-1:2021)
Leder - Bestimmung von chlorierten Kohlenwasserstoffen in Leder - Teil 1:
Chromatographisches Verfahren für kurzkettige Chlorparaffine (SCCP) (ISO 18219-
1:2021)
Cuir - Dosage des hydrocarbures chlorés dans le cuir - Partie 1: Méthode
chromatographique pour les paraffines chlorées à chaîne courte (PCCC) (ISO 18219-
1:2021)
Ta slovenski standard je istoveten z: EN ISO 18219-1:2021
ICS:
59.140.30 Usnje in krzno Leather and furs
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 18219-1
EUROPEAN STANDARD
NORME EUROPÉENNE
June 2021
EUROPÄISCHE NORM
ICS 59.140.30 Supersedes EN ISO 18219:2015
English Version
Leather - Determination of chlorinated hydrocarbons in
leather - Part 1: Chromatographic method for short-chain
chlorinated paraffins (SCCPs) (ISO 18219-1:2021)
Cuir - Dosage des hydrocarbures chlorés dans le cuir - Leder - Bestimmung von chlorierten
Partie 1: Méthode chromatographique pour les Kohlenwasserstoffen in Leder - Teil 1:
paraffines chlorées à chaîne courte (PCCC) (ISO 18219- Chromatographisches Verfahren für kurzkettige
1:2021) Chlorparaffine (SCCP) (ISO 18219-1:2021)
This European Standard was approved by CEN on 7 May 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18219-1:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 18219-1:2021) has been prepared by Technical Committee ISO/IULTCS
"International Union of Leather Technologists and Chemists Societies" in collaboration with Technical
Committee CEN/TC 289 “Leather” the secretariat of which is held by UNI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by December 2021, and conflicting national standards
shall be withdrawn at the latest by December 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 18219:2015.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 18219-1:2021 has been approved by CEN as EN ISO 18219-1:2021 without any
modification.
INTERNATIONAL ISO
STANDARD 18219-1
IULTCS
IUC 30-1
First edition
2021-05
Leather — Determination of
chlorinated hydrocarbons in
leather —
Part 1:
Chromatographic method for short-
chain chlorinated paraffins (SCCPs)
Cuir — Dosage des hydrocarbures chlorés dans le cuir —
Partie 1: Méthode chromatographique pour les paraffines chlorées à
chaîne courte (PCCC)
Reference numbers
ISO 18219-1:2021(E)
IULTCS/IUC 30-1:2021(E)
©
ISO 2021
ISO 18219-1:2021(E)
IULTCS/IUC 30-1:2021(E)
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2021 – All rights reserved

ISO 18219-1:2021(E)
IULTCS/IUC 30-1:2021(E)
Contents Page
Foreword .iv
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Apparatus and materials. 1
6 Reagents . 2
7 Sampling . 2
8 Sample preparation and analysis . 2
8.1 Preparation of the SCCPs calibration solution (50 µg/ml) with 59 % chlorine content . 2
8.2 Extraction of leather . 3
8.3 Sulfuric acid clean-up . 3
8.4 GC-ECNI-MS determination . 3
9 Expression of results . 3
9.1 Evaluation . 3
9.2 Ions used for quantification . 3
9.3 Interference to SCCPs determination . 3
9.4 Calculation . 4
9.5 Precision . 4
10 Test report . 4
Annex A (informative) Gas chromatographic (GC-ECNI-MS) analysis operating parameters .6
Annex B (informative) Liquid chromatographic (LC-MS/MS) analysis operating parameters .7
Annex C (informative) Integration using peak shape evaluation (PSE) with GC-ECNI-MS .10
Bibliography .13
ISO 18219-1:2021(E)
IULTCS/IUC 30-1:2021(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document prepared by the Chemical Test Commission of the International Union of Leather
Technologists and Chemists Societies (IUC Commission, IULTCS) in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 289, Leather, the secretariat of
which is held by UNI, in accordance with the agreement on technical co-operation between ISO and CEN
(Vienna Agreement).
IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to
further the advancement of leather science and technology. IULTCS has three Commissions, which
are responsible for establishing international methods for the sampling and testing of leather. ISO
recognizes IULTCS as an international standardizing body for the preparation of test methods for
leather.
This first edition of ISO 18219-1 cancels and replaces ISO 18219:2015, which has been technically
revised.
The main changes to ISO 18219:2015 are as follows:
— a modification of the ISO number and the title;
— Introduction revised and updated;
— a new Clause 3;
— technical changes to Clause 4;
— in 6.2, a new internal standard;
— in 8.3, the SPE clean-up changed to a procedure using sulfuric acid;
— new 9.2, 9.3, 9.4 and 9.5 to improve the method;
— a new Annex B with a LC-MS/MS procedure;
iv © ISO 2021 – All rights reserved

ISO 18219-1:2021(E)
IULTCS/IUC 30-1:2021(E)
— a new Annex C explaining how to interpret the chromatograms with peak shape evaluation (PSE).
A list of all parts in the ISO 18219 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
ISO 18219-1:2021(E)
IULTCS/IUC 30-1:2021(E)
Introduction
Short-chain chlorinated paraffins (SCCPs) are a mixture of chlorinated hydrocarbons with a chain
length of 10 to 13 carbon atoms and a chlorine content of 40 % to 70 %. SCCPs are classified as
dangerous to the environment, since they are very toxic to aquatic organisms and can cause long-term
adverse effects in the aquatic environment.
In 2002, the European Directive 2002/45/EC restricted the sale and use of SCCPs (C to C ) in product
10 13
preparations for the fatliquoring of leather. Preparations containing concentrations equal to or higher
than 1 % of SCCPs were forbidden. This directive is included as part of the EU Regulation 1907/2006
(REACH). Within this EU Regulation, in October 2008, the SCCPs were added to the candidate list of
[2]
substances of very high concern (SVHC) .
[3]
The EU Commission Regulation 2015/2030 in November 2015 prohibited alkanes C to C , chloro
10 13
(SCCPs) (CAS No 85535-84-8), as constituents of articles. Articles containing SCCPs in concentrations
lower than 0,15 % by mass are allowed.
In 2017, the SCCPs were listed in Annex A of the Stockholm Convention on Persistent Organic Pollutants
[4]
(POP) .
The analysis of chlorinated paraffins is a challenge. The technical compounds are mixtures of up to
200 congeners with different chain lengths and degrees of chlorination. GC chromatograms of these
complex mixtures typically show a lot of overlapping peaks that can be difficult to separate. In
particular, the responses to the various chlorination degrees can vary over a large range.
In addition, the presence of sulfochlorinated paraffins and equivalent chain-length chloroalkenes in
such technical compounds can cause interference.
This document describes a procedure to compare the chromatogram results for SCCPs compounds from
a test sample with the chromatogram results of a defined calibration standard of the most typically
used mixture (59 % chlorination for SCCPs). With this gas chromatography negative ion chemical
ionization mass spectrometry (GC-ECNI-MS) procedure it uses four ion traces for identifying the SCCPs.
vi © ISO 2021 – All rights reserved

ISO 18219-1:2021(E)
INTERNATIONAL STANDARD
IULTCS/IUC 30-1:2021(E)
Leather — Determination of chlorinated hydrocarbons in
leather —
Part 1:
Chromatographic method for short-chain chlorinated
paraffins (SCCPs)
1 Scope
This document specifies a chromatographic method to determine the amount of short-chain chlorinated
paraffins (SCCPs) C to C in processed and unprocessed leathers.
10 13
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 2418, Leather — Chemical, physical and mechanical and fastness tests — Sampling location
ISO 4044, Leather — Chemical
...