EN ISO 13365-1:2020
(Main)Leather - Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography - Part 1: Acetonitrile extraction method (ISO 13365-1:2020, Corrected version 2021-03)
General Information
- Abstract
This document specifies a test method by acetonitrile solvent extraction for the determination of the total content (solvent extractible) of the following preservative agents in leather by liquid chromatography:
— 2-(thiocyanomethylthio)-benzothiazole (TCMTB);
— 4-chloro-3-methylphenol (PCMC);
— 2-phenylphenol (OPP);
— 2-octylisothiazol-3(2H)-one (OIT);
This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.
- Status
- Published
- Publication Date
- 08-Sep-2020
- Withdrawal Date
- 30-Mar-2021
- Technical Committee
- CEN/TC 289 - Leather
- Drafting Committee
- CEN/TC 289/WG 1 - Chemical test methods
- Current Stage
- 6060 - Definitive text made available (DAV) - Publishing
- Start Date
- 09-Sep-2020
- Completion Date
- 09-Sep-2020
Overview
EN ISO 13365-1:2020 (Corrected 2021-03) specifies a standardized laboratory method for the chemical determination of preservative agents in leather using acetonitrile solvent extraction followed by liquid chromatography (HPLC) with UV or mass spectrometric (MS) detection. The method targets the total solvent‑extractible content of four common leather preservatives: TCMTB, PCMC, OPP, and OIT, and can also detect their breakdown products. This standard is published by CEN/ISO and supersedes EN ISO 13365:2011.
Key topics and technical requirements
- Scope: Determination of 2-(thiocyanomethylthio)-benzothiazole (TCMTB), 4-chloro-3-methylphenol (PCMC), 2-phenylphenol (OPP) and 2-octylisothiazol-3(2H)-one (OIT) in leather.
- Sample preparation: Sampling per ISO 2418; sample preparation per ISO 4044. Wet intermediates must be dried before testing.
- Extraction: ~1 g leather extracted with 20 ml acetonitrile in an ultrasonic bath (≈40 kHz) for 1 h ± 5 min at room temperature (mixture may reach ~35 °C).
- Filtration/clarification: Optional membrane filtration (polyamide, 0.45 µm) or centrifugation of extracts.
- Chromatography: HPLC with UV or MS detection using reversed‑phase C8 or C18 columns (pre‑column recommended). Annex A provides recommended chromatographic conditions and MS options.
- Reagents & purity: HPLC-grade acetonitrile; deionized water (ISO 3696 grade 3); analytical/LC‑MS grade solvents and standards. Target compound standards specified (≥97–99.7% purity).
- Calibration: At least four calibration solutions prepared in acetonitrile (range examples: 2 to 100 mg/L) to produce linear calibration of peak area vs concentration. Store calibration solutions at (4 ± 3) °C.
- Reporting: Calculate total solvent‑extractible content and record sampling, extraction and chromatographic parameters.
Applications and users
- Quality control and routine testing in tanneries and leather finishing operations
- Independent analytical laboratories performing regulatory or supplier compliance testing
- Product safety and environmental teams assessing preservative residues and potential breakdown products
- Research groups studying preservative behavior, microbiological protection, or leather chemistry
Related standards
- ISO 13365 series (Part 2 complements extraction with alternative methods)
- ISO 2418 - sampling location for leather
- ISO 4044 - preparation of chemical test samples
- ISO 3696 - laboratory water quality
- ISO 4684 - determination of volatile matter
Keywords: EN ISO 13365-1:2020, leather preservative analysis, acetonitrile extraction, HPLC, LC-MS, TCMTB, PCMC, OPP, OIT, leather testing, chemical determination.
Relations
- Effective Date
- 08-Jun-2022
- Effective Date
- 25-Aug-2026
- Effective Date
- 09-Feb-2026
- Effective Date
- 09-Feb-2026
- Refers
ISO 2418:2017 - Leather — Chemical, physical and mechanical and fastness tests — Sampling location - Effective Date
- 09-Feb-2026
- Effective Date
- 28-Jan-2026
- Effective Date
- 12-Feb-2026
Get Certified
Connect with accredited certification bodies for this standard
Control Union Certifications
Global certification for agriculture and sustainability.
Hohenstein Institut
Textile testing and certification.
Bureau Veritas Bangladesh
Bureau Veritas certification services in Bangladesh.
Sponsored listings
Frequently Asked Questions
EN ISO 13365-1:2020 is a standard published by the European Committee for Standardization (CEN). Its full title is "Leather - Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography - Part 1: Acetonitrile extraction method (ISO 13365-1:2020, Corrected version 2021-03)". This standard covers: This document specifies a test method by acetonitrile solvent extraction for the determination of the total content (solvent extractible) of the following preservative agents in leather by liquid chromatography: — 2-(thiocyanomethylthio)-benzothiazole (TCMTB); — 4-chloro-3-methylphenol (PCMC); — 2-phenylphenol (OPP); — 2-octylisothiazol-3(2H)-one (OIT); This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.
This document specifies a test method by acetonitrile solvent extraction for the determination of the total content (solvent extractible) of the following preservative agents in leather by liquid chromatography: — 2-(thiocyanomethylthio)-benzothiazole (TCMTB); — 4-chloro-3-methylphenol (PCMC); — 2-phenylphenol (OPP); — 2-octylisothiazol-3(2H)-one (OIT); This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.
EN ISO 13365-1:2020 is classified under the following ICS (International Classification for Standards) categories: 59.140.30 - Leather and furs. The ICS classification helps identify the subject area and facilitates finding related standards.
EN ISO 13365-1:2020 has the following relationships with other standards: It is inter standard links to EN ISO 13365:2011, ISO 4684:2005, ISO 4044:2017, ISO 3696:1987, ISO 2418:2017, EN 1457-1:2012, ISO 13365-1:2020. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
EN ISO 13365-1:2020 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
01-november-2020
Nadomešča:
SIST EN ISO 13365:2011
Usnje - Kemijsko določevanje sredstev za zaščito (TCMTB, PCMC, OPP, OIT) v
usnju s tekočinsko kromatografijo - 1. del: Metoda ekstrakcije acetonitrila (ISO
13365-1:2020)
Leather - Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT)
content in leather by liquid chromatography - Part 1: Acetonitrile extraction method (ISO
13365-1:2020)
Leder - Chemische Prüfungen - Bestimmung von Konservierungsmitteln (TCMTB, CMK,
OPP, OIT) in Leder mittels Flüssigchromatographie - Teil 1: Gesamtanteil (ISO 13365-
1:2020)
Cuir - Dosage chimique des agents de conservation (TCMTB, PCMC, OPP, OIT) dans le
cuir par chromatographie en phase liquide - Partie 1: Extraction à l’acétonitrile (ISO
13365-1:2020)
Ta slovenski standard je istoveten z: EN ISO 13365-1:2020
ICS:
59.140.30 Usnje in krzno Leather and furs
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 13365-1
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2020
EUROPÄISCHE NORM
ICS 59.140.30 Supersedes EN ISO 13365:2011
English Version
Leather - Chemical determination of the preservative
(TCMTB, PCMC, OPP, OIT) content in leather by liquid
chromatography - Part 1: Acetonitrile extraction method
(ISO 13365-1:2020)
Cuir - Dosage chimique des agents de conservation Leder - Chemische Prüfungen - Bestimmung von
(TCMTB, PCMC, OPP, OIT) dans le cuir par Konservierungsmitteln (TCMTB, CMK, OPP, OIT) in
chromatographie en phase liquide - Partie 1: Leder mittels Flüssigchromatographie - Teil 1:
Extraction à l'acétonitrile (ISO 13365-1:2020) Gesamtanteil (ISO 13365-1:2020)
This European Standard was approved by CEN on 2 July 2020.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 13365-1:2020 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 13365-1:2020) has been prepared by Technical Committee IULTCS
"International Union of Leather Technologists and Chemists Societies" in collaboration with Technical
Committee CEN/TC 289 “Leather” the secretariat of which is held by UNI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2021, and conflicting national standards shall
be withdrawn at the latest by March 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 13365:2011.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 13365-1:2020 has been approved by CEN as EN ISO 13365-1:2020 without any
modification.
INTERNATIONAL ISO
STANDARD 13365-1
IULTCS
IUC 29-1
First edition
2020-07
Leather — Chemical determination
of the preservative (TCMTB, PCMC,
OPP, OIT) content in leather by liquid
chromatography —
Part 1:
Acetonitrile extraction method
Cuir — Dosage chimique des agents de conservation (TCMTB, PCMC,
OPP, OIT) dans le cuir par chromatographie en phase liquide —
Partie 1: Extraction à l’acétonitrile
Reference numbers
ISO 13365-1:2020(E)
IULTCS/IUC 29-1:2020(E)
©
ISO 2020
ISO 13365-1:2020(E)
IULTCS/IUC 29-1:2020(E)
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved
ISO 13365-1:2020(E)
IULTCS/IUC 29-1:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
5.1 General . 2
5.2 Target compounds . 2
5.3 Preparation of calibration solutions . 2
6 Apparatus and materials. 3
7 Testing procedure . 3
7.1 Sampling and sample preparation . 3
7.2 Extraction procedure . 3
7.3 Analytical procedure . 3
8 Calculation . 3
9 Test report . 4
Annex A (informative) Chromatographic conditions . 5
ISO 13365-1:2020(E)
IULTCS/IUC 29-1:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade
...