prEN ISO 10519
(Main)Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO/DIS 10519:2026)
- Abstract
ISO 10519:2015 specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.
- Status
- Not Published
- Publication Date
- 22-Nov-2027
- Current Stage
- 4060 - Closure of enquiry - Enquiry
- Start Date
- 26-Aug-2026
- Completion Date
- 26-Aug-2026
prEN ISO 10519 is the CEN draft standard aligned with ISO/FDIS 10519:2026 for measuring chlorophyll content in rapeseed by spectrometric analysis. It specifies how to prepare the seed sample, extract the pigments, measure absorbance, and calculate the result in milligrams per kilogram. It is aimed at laboratories and quality-control teams that need comparable results for rapeseed samples, not oils.
What does prEN ISO 10519 specify?
prEN ISO 10519 specifies a spectrometric method for determining the chlorophyll content of rapeseed. The method is explicitly not for chlorophyll in oils, so it is intended for seed analysis rather than oil testing.
The document is organized into Clauses 1 to 12, followed by two informative annexes and a bibliography.
| Annex | What it covers |
|---|---|
| Annex A | Results of an interlaboratory test and precision data |
| Annex B | Preparation of the calibration curve to determine the k factor |
What are the key requirements of prEN ISO 10519?
The method starts with representative sampling and sample preparation, because the result depends on using a laboratory sample that has not been damaged in transport and storage.
Sample preparation and test portion
- Clause 7 says sampling is outside the method itself, and points to ISO 542 for a recommended sampling method. In practice, the laboratory must receive a representative sample.
- Clause 8 requires preparation of the test sample in accordance with ISO 664 after impurities are removed. If moisture is above a mass fraction of 10 %, the seeds are dried at 45 °C for 12 h to reduce pigment loss. This matters because excess moisture can affect extraction and chlorophyll stability.
- Clause 9.1 requires a 2 g test portion weighed to the nearest 0,001 g. This keeps the result tied to a defined, repeatable sample mass.
Extraction and measurement
- Clause 5.1 specifies the extraction solvent as anhydrous ethanol combined with anhydrous iso-octane, anhydrous technical n-heptane, or anhydrous petroleum ether. Clause 6.3 then uses that solvent with a mechanical microgrinder or ball mill. In practice, the analyst needs dry reagents and a consistent extraction setup.
- Clause 9.2 gives two extraction options: 1 h shaking in a tube with steel balls, or 20 min in a commercial ball mill with at least four medium-sized steel balls. After extraction, the solution is allowed to settle for 10 min and filtered before measurement.
- Clause 9.3 requires absorbance readings at 665 nm, 705 nm and 625 nm with a spectrometer suitable for 600 nm to 700 nm and a spectral bandwidth of 2 nm. The 705 nm and 625 nm readings are used for baseline correction, so the result is based on corrected absorbance rather than a single raw reading.
Calculation, calibration and precision
- Clause 10 expresses chlorophyll content in milligrams per kilogram using the corrected absorbance, path length, test portion mass and solvent volume. The document uses k = 13, but also says k may need to be determined if the spectrometer bandwidth is variable. Annex B shows how to prepare the calibration curve for that purpose.
- Clause 11 gives the precision statements: repeatability and reproducibility limits are based on the interlaboratory study in Annex A. In practice, these values tell users how close two results should be when the method is run within one lab or between different labs.
- Clause 12 lists what the test report must include, such as the sampling method if known, the method used, the result, any final quoted result after repeatability checking, the test date, and any optional details or incidents that could affect the result.
What terms does prEN ISO 10519 define?
prEN ISO 10519 defines chlorophyll content and uses several measurement terms that are central to the method.
- Chlorophyll content - the mass fraction of substances in the sample that absorb near 665 nm, expressed as chlorophyll A in milligrams per kilogram.
- Test portion - the 2 g portion of prepared seed that is actually extracted and measured.
- Corrected absorbance - the absorbance value adjusted using the readings at 705 nm and 625 nm to reduce baseline effects.
- Repeatability - the closeness of two results produced in the same laboratory under the same operating conditions.
- Reproducibility - the closeness of results produced in different laboratories under different operating conditions.
- k factor - the calibration constant used in the calculation; Annex B shows how to determine it when needed.
- Bandwidth - the spectrometer setting that affects measurement quality and, in some cases, the value of k.
Who uses prEN ISO 10519?
prEN ISO 10519 is used by laboratories that test rapeseed for chlorophyll content and need results that can be compared across equipment and sites. It is also relevant to quality managers and technical staff in the oilseed sector who prepare test reports, check method precision, or validate spectrometers and extraction procedures. Because the document includes precision data and calibration guidance, it is also useful for method-development and laboratory-accreditation work.
What changed in prEN ISO 10519 from the previous edition?
prEN ISO 10519 replaces ISO 10519:2015. The foreword says this fourth edition is a minor revision, incorporates ISO 10519:2015/Amd 1:2025, and adds Annex B, which covers preparation of the calibration curve to determine the k factor.
Which standards are used with prEN ISO 10519?
prEN ISO 10519 refers to several standards that support sampling, sample preparation, moisture correction, and precision work.
- ISO 648 - provides the requirements for the class A 30 ml pipettes used in the method.
- ISO 664 - gives the method for reducing a laboratory sample to a test sample.
- ISO 665 - is used when chlorophyll content is reported relative to the dry product, because it supplies the moisture determination needed for that calculation.
- ISO 542 - is cited as a recommended sampling method in Clause 7.
- ISO 5725:1986 - is cited in Annex A for the interlaboratory test behind the precision data.
What does the prEN ISO 10519 document contain?
prEN ISO 10519 contains the full workflow for the spectrometric determination, from sample preparation through reporting. Clause 6 lists the apparatus, including the mechanical microgrinder shown in Figure 1 and the spectrometer requirements. Clause 10 gives the calculation based on corrected absorbance, and Annex B adds a calibration procedure for determining k when the spectrometer bandwidth makes the default constant unsuitable.
Annex A provides interlaboratory precision results in Table A.1, including repeatability and reproducibility statistics. This makes the document useful not only for routine testing, but also for checking whether a laboratory’s results fit the expected performance of the method.
Relations
- Consolidated By
ISO 10519:2026 - Rapeseed — Determination of chlorophyll content — Spectrometric method - Effective Date
- 18-Apr-2026
- Effective Date
- 08-Apr-2026
- Effective Date
- 08-Apr-2026
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Frequently Asked Questions
prEN ISO 10519 is a draft published by the European Committee for Standardization (CEN). Its full title is "Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO/DIS 10519:2026)". This standard covers: ISO 10519:2015 specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.
ISO 10519:2015 specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.
prEN ISO 10519 is classified under the following ICS (International Classification for Standards) categories: 67.200.20 - Oilseeds. The ICS classification helps identify the subject area and facilitates finding related standards.
prEN ISO 10519 has the following relationships with other standards: It is inter standard links to ISO 10519:2026, EN ISO 10519:2015/A1:2025, EN ISO 10519:2015. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
prEN ISO 10519 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2026
Seme oljne repice - Določevanje klorofila - Spektrometrijska metoda (ISO/FDIS
10519:2026)
Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO/FDIS
10519:2026)
Rapssamen - Bestimmung des Chlorophyllgehaltes - Spektrometrisches Verfahren
(ISO/FDIS 10519:2026)
Graines de colza - Détermination de la teneur en chlorophylle - Méthode
spectrométrique (ISO/FDIS 10519:2026)
Ta slovenski standard je istoveten z: prEN ISO 10519
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
FINAL DRAFT
International
Standard
ISO/FDIS 10519
ISO/TC 34/SC 2
Rapeseed — Determination
Secretariat: AFNOR
of chlorophyll content —
Voting begins on:
Spectrometric method
2026-06-03
Graines de colza — Détermination de la teneur en chlorophylle —
Voting terminates on:
Méthode spectrométrique
2026-08-26
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
ISO/CEN PARALLEL PROCESSING LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 10519:2026(en) © ISO 2026
FINAL DRAFT
ISO/FDIS 10519:2026(en)
International
Standard
ISO/FDIS 10519
ISO/TC 34/SC 2
Rapeseed — Determination
Secretariat: AFNOR
of chlorophyll content —
Voting begins on:
Spectrometric method
Graines de colza — Détermination de la teneur en chlorophylle —
Voting terminates on:
Méthode spectrométrique
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2026
IN ADDITION TO THEIR EVALUATION AS
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BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
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Published in Switzerland Reference number
ISO/FDIS 10519:2026(en) © ISO 2026
ii
ISO/FDIS 10519:2026(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagent . 1
6 Apparatus . 2
7 Sampling . 2
8 Preparation of test sample . 3
9 Procedure . 3
9.1 Test portions .3
9.2 Extraction .3
9.3 Determination .3
10 Expression of results . 4
11 Precision . 4
11.1 Repeatability .4
11.2 Reproducibility .4
12 Test report . 4
Annex A (informative) Results of interlaboratory test . 6
Annex B (informative) Preparation of the calibration curve to determine the k factor . 7
Bibliography . 9
iii
ISO/FDIS 10519:2026(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
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with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
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patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
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This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 2, Oleaginous seeds and fruits and oilseed meals, in collaboration with the European Committee for
Standardization (CEN) Technical Committee CEN/TC 307, Oilseeds, vegetable and animal fats and oils and their
by-products - Methods of sampling and analysis, in accordance with the Agreement on technical cooperation
between ISO and CEN (Vienna Agreement).
This fourth edition cancels and replaces the third edition (ISO 10519:2015), of which it constitutes a minor
revision. It also incorporates the Amendment ISO 10519:2015/Amd 1:2025.
The changes are as follows:
— Annex B “Preparation of the calibration curve to determine the k factor” has been added.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
FINAL DRAFT International Standard ISO/FDIS 10519:2026(en)
Rapeseed — Determination of chlorophyll content —
Spectrometric method
1 Scope
This document specifies a spectrometric method for the determination of the chlorophyll content of
rapeseed. It is not applicable to the determination of chlorophyll in oils.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 648, Laboratory glassware — Single-volume pipettes
ISO 664, Oilseeds — Reduction of laboratory sample to test sample
ISO 665, Oilseeds — Determination of moisture and volatile matter content
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
chlorophyll content
mass fraction of substances in the sample contributing to the absorption band at a wavelength near 665 nm,
measured as chlorophyll A
Note 1 to entry: The chlorophyll conten
...