This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke. The smoking of cigarettes and the collection of mainstream smoke are carried out according to ISO 4387.
NOTE ISO 20778 and ISO 22253 provide the determination method of nicotine in smoke with an intense smoking regime.

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This document specifies methods for the determination of total particulate matter and for the subsequent determination of nicotine-free dry particulate matter present in the smoke from cigarettes generated and collected using a routine analytical smoking machine.

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ISO 8243:2013 specifies two methods of providing representative samples of a population of cigarettes manufactured for sale. Different procedures are specified according to whether sampling is undertaken at the point of sale or at a factory.
Sampling "at one point in time" provides for appraisal of the chosen properties of the cigarettes on that occasion. Sampling is carried out within as short a period as possible.
Sampling "over a period of time" provides for on-going appraisals. It can be considered for practical purposes as a series of samples each taken "at one point in time".
ISO 8243:2013 provides information on the statistical basis for the treatment of data and gives estimates, based on practical experience, of the typical confidence intervals for nicotine-free dry particulate matter (NFDPM), nicotine and CO yields which may be found when a product is sampled in accordance with ISO 8243:2013 and smoked in accordance with the procedures specified in ISO 3308, ISO 3402, ISO 4387, ISO 8454, ISO 10315, ISO 10362‑1 and ISO 10362‑2.

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ISO 8454:2007 specifies a method for the determination of carbon monoxide (CO) in the vapour phase of cigarette smoke.

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