CEN/TC 454/WG 6 - Product test methods

This document specifies a laboratory method for the determination of chlorophyll a content in algae. The method was initially tested and evaluated on the microalgae species Nannochloropsis sp. and a heat treated algal product tomato soup with Nannochloropsis sp. supplement, and the macro algae species Ulva sp., Furcellaria lumbricalis, and Saccharina latissima. During an Interlaboratory Trial the method was tested on the microalgae species Nannochloropsis sp. and the macro algae species Saccharina latissima. The microalgae species Nannochloropsis sp. and Phaodactlylum sp. and the macro algae species Ulva sp. and Saccharina latissima were tested in a Round Robin test. This document is only validated for chlorophyll a, but it can be used for other chlorophylls as well.

This document specifies the sample preparation of dry and wet samples of algae and algae products. This document enables laboratories analysing algae samples to report accurate dry weight percentages and to obtain representative samples possible for further examination.

This European standard specifies a laboratory method for the determination of the
carotenoid  content in microalgae. The method is based on the development of the
chlorophyll a standard, which resulted in prEN 18034. This method has been validated
for the microalgae species  Nannochloropsis and Phaodactlylum. This standard is only
validated for fucoxanthin, beta carotene and lutein content, but could be used for other
carotenoids as well. Given small adaptations to be able to measure smaller
concentrations, this method could also be used for macro algae.
This standard is developed in CEN/TC 454/WG 6 Product test methods.

This document specifies a laboratory method for the quantification of phycocyanin content in the genus Arthrospira (Spirulina) by a
spectrophotometric method.