SIST EN ISO 6865:2001
(Main)Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)
Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)
Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg.
NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
Futtermittel - Bestimmung des Rohfasergehaltes - Verfahren mit Zwischenfiltration (ISO/FDIS 6865:2000)
Diese Internationale Norm legt ein Verfahren zur Bestimmung des Rohfasergehaltes mit Zwischenfiltration fest. Es wird sowohl ein manuelles Verfahren als auch ein halbautomatisches Verfahren beschrieben. Dieses Verfahren ist auf Futtermittel mit einem Rohfasergehalt von größer als 10 g/kg anwendbar. ANMERKUNG Für Futtermittel mit einem Rohfasergehalt kleiner als oder gleich 10 g/kg darf das in ISO 6541 : 1981 beschriebene Verfahren angewandt werden. Diese Internationale Norm ist auch auf Getreide und Hülsenfrüchte anwendbar.
Aliments des animaux - Détermination de la teneur en cellulose brute - Méthode avec filtration intermédiaire (ISO/FDIS 6865:2000)
Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg.
NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
Krmila - Določevanje surovih vlaken - Metoda z vmesno filtracijo (ISO 6865:2000)
General Information
- Status
- Published
- Publication Date
- 31-Jan-2001
- Technical Committee
- KŽP - Agricultural food products
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 01-Feb-2001
- Due Date
- 01-Feb-2001
- Completion Date
- 01-Feb-2001
Relations
- Effective Date
- 09-Feb-2026
Overview
SIST EN ISO 6865:2001 is a key international standard that defines the procedure for determining the crude fibre content in animal feeding stuffs using a method with intermediate filtration. This standard, technically identical to ISO 6865:2000, is essential for laboratories and manufacturers in the animal feed and agriculture sectors. It describes both a manual and a semi-automatic procedure, making it adaptable to different laboratory environments. The method is applicable to animal feeds with a crude fibre content greater than 10 g/kg, as well as to cereals and legumes. For lower fibre contents (≤ 10 g/kg), reference is made to ISO 6541.
Key Topics
Scope and Applicability
- Applicable to animal feeds, cereals, and legumes with crude fibre content >10 g/kg.
- Provides both manual and semi-automatic procedures, allowing flexibility in implementation.
- Highlights the use of ISO 6541 for low-fibre samples.
Testing Methodology
- Utilizes intermediate filtrations after acid and alkaline digestion.
- Outlines operational steps for both manual and semi-automatic approaches.
- Details necessary laboratory apparatus and reagents for precise determination.
- Includes requirements for sample preparation and procedural blanks.
Precision and Reporting
- Specifies procedures to ensure repeatability and reproducibility of results across laboratories.
- Provides guidance on documentation and reporting for test results, including sample identification and procedural notes.
Applications
The standardized determination of crude fibre content is critical for several practical purposes:
Nutritional Labeling
Enables feed producers to accurately declare fibre content, ensuring regulatory compliance and transparency for end-users.Quality Control in Feed Manufacturing Routine analysis of crude fibre is fundamental for quality assurance during feed formulation, helping to maintain product consistency and efficiency.
Regulatory Compliance Laboratories and manufacturers use this method to adhere to international and regional feed regulations, supporting safe and standardized animal nutrition.
Research and Product Development Used in animal nutrition research for the development and comparison of new feed products, and for studies of feed digestibility and animal health.
Testing Cereals and Pulses Beyond compound feeds, the method is applicable for the analysis of cereals and legumes, supporting broader agricultural quality control.
Related Standards
For comprehensive feed analysis and quality management, the following international standards should also be considered:
- ISO 6541: For determination of crude fibre content in feeds with ≤ 10 g/kg crude fibre (Modified Scharrer method).
- ISO 6492: Animal feeding stuffs - Determination of fat content.
- ISO 6497: Sampling of animal feeding stuffs.
- ISO 6498: Preparation of test samples for animal feeding stuffs.
- ISO 4793: Laboratory sintered (fritted) filters - Porosity grading.
- ISO 3696: Water for analytical laboratory use - Specification and test methods.
Summary
SIST EN ISO 6865:2001 provides an authoritative and harmonized method for the determination of crude fibre content in animal feeding stuffs, cereals, and pulses. By laying out precise procedures and equipment requirements, it ensures accuracy in feed analysis, supports quality control, and strengthens regulatory compliance-making it indispensable for feed manufacturers, quality assurance laboratories, and agricultural research institutions worldwide.
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Frequently Asked Questions
SIST EN ISO 6865:2001 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)". This standard covers: Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg. NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg. NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
SIST EN ISO 6865:2001 is classified under the following ICS (International Classification for Standards) categories: 65.120 - Animal feeding stuffs. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 6865:2001 has the following relationships with other standards: It is inter standard links to SIST ISO 3696:1995. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
SIST EN ISO 6865:2001 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Futtermittel - Bestimmung des Rohfasergehaltes - Verfahren mit Zwischenfiltration (ISO/FDIS 6865:2000)Aliments des animaux - Détermination de la teneur en cellulose brute - Méthode avec filtration intermédiaire (ISO/FDIS 6865:2000)Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN ISO 6865:2000SIST EN ISO 6865:2001en01-februar-2001SIST EN ISO 6865:2001SLOVENSKI
STANDARD
ReferencenumberISO6865:2000(E)©ISO2000INTERNATIONALSTANDARDISO6865Firstedition2000-10-15Animalfeedingstuffs—Determinationofcrudefibrecontent—MethodwithintermediatefiltrationAlimentsdesanimaux—Déterminationdelateneurencellulosebrute—MéthodeavecfiltrationintermédiaireSIST EN ISO 6865:2001
ISO6865:2000(E)PDFdisclaimerThisPDFfilemaycontainembeddedtypefaces.InaccordancewithAdobe'slicensingpolicy,thisfilemaybeprintedorviewedbutshallnotbeeditedunlessthetypefaceswhichareembeddedarelicensedtoandinstalledonthecomputerperformingtheediting.Indownloadingthisfile,partiesacceptthereintheresponsibilityofnotinfringingAdobe'slicensingpolicy.TheISOCentralSecretariatacceptsnoliabilityinthisarea.AdobeisatrademarkofAdobeSystemsIncorporated.DetailsofthesoftwareproductsusedtocreatethisPDFfilecanbefoundintheGeneralInforelativetothefile;thePDF-creationparameterswereoptimizedforprinting.EverycarehasbeentakentoensurethatthefileissuitableforusebyISOmemberbodies.Intheunlikelyeventthataproblemrelatingtoitisfound,pleaseinformtheCentralSecretariatattheaddressgivenbelow.©ISO2000Allrightsreserved.Unlessotherwisespecified,nopartofthispublicationmaybereproducedorutilizedinanyformorbyanymeans,electronicormechanical,includingphotocopyingandmicrofilm,withoutpermissioninwritingfromeitherISOattheaddressbeloworISO'smemberbodyinthecountryoftherequester.ISOcopyrightofficeCasepostale56CH-1211Geneva20Tel.+41227490111Fax+41227490947E-mailcopyright@iso.chWebwww.iso.chPrintedinSwitzerlandii©ISO2000–AllrightsreservedSIST EN ISO 6865:2001
ISO6865:2000(E)©ISO2000–AllrightsreservediiiForewordISO(theInternationalOrganizationforStandardization)isaworldwidefederationofnationalstandardsbodies(ISOmemberbodies).TheworkofpreparingInternationalStandardsisnormallycarriedoutthroughISOtechnicalcommittees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeenestablishedhastherighttoberepresentedonthatcommittee.Internationalorganizations,governmentalandnon-governmental,inliaisonwithISO,alsotakepartinthework.ISOcollaboratescloselywiththeInternationalElectrotechnicalCommission(IEC)onallmattersofelectrotechnicalstandardization.InternationalStandardsaredraftedinaccordancewiththerulesgivenintheISO/IECDirectives,Part3.DraftInternationalStandardsadoptedbythetechnicalcommitteesarecirculatedtothememberbodiesforvoting.PublicationasanInternationalStandardrequiresapprovalbyatleast75%ofthememberbodiescastingavote.AttentionisdrawntothepossibilitythatsomeoftheelementsofthisInternationalStandardmaybethesubjectofpatentrights.ISOshallnotbeheldresponsibleforidentifyinganyorallsuchpatentrights.InternationalStandardISO6865waspreparedbyTechnicalCommitteeISO/TC34,Foodproducts,SubcommitteeSC10,Animalfeedingstuffs.AnnexAofthisInternationalStandardisforinformationonly.SIST EN ISO 6865:2001
INTERNATIONALSTANDARDISO6865:2000(E)©ISO2000–Allrightsreserved1Animalfeedingstuffs—Determinationofcrudefibrecontent—Methodwithintermediatefiltration1ScopeThisInternationalStandardspecifiesamethodwithintermediatefiltrationforthedeterminationofthecrudefibrecontent.Amanualprocedureandasemi-automaticprocedurearedescribed.Themethodisapplicabletoanimalfeedingstuffswithacrudefibrecontentgreaterthan10g/kg.NOTEForanimalfeedingstuffswithacrudefibrecontentequaltoorlessthan10g/kg,themethoddescribedinISO6541[7]maybeused.ThisInternationalStandardisalsoapplicabletocerealsandpulses.2NormativereferencesThefollowingnormativedocumentscontainprovisionswhich,throughreferenceinthistext,constituteprovisionsofthisInternationalStandard.Fordatedreferences,subsequentamendmentsto,orrevisionsof,anyofthesepublicationsdonotapply.However,partiestoagreementsbasedonthisInternationalStandardareencouragedtoinvestigatethepossibilityofapplyingthemostrecenteditionsofthenormativedocumentsindicatedbelow.Forundatedreferences,thelatesteditionofthenormativedocumentsreferredtoapplies.MembersofISOandIECmaintainregistersofcurrentlyvalidInternationalStandards.ISO3696:1987,Waterforanalyticallaboratoryuse—Specificationandtestmethods.ISO6498:1998,Animalfeedingstuffs—Preparationoftestsamples.3TermanddefinitionForthepurposesofthisInternationalStandard,thefollowingtermanddefinitionapply.3.1crudefibrecontentlossinmassresultingfromashingofthedriedresidueobtainedafteracidandalkalinedigestionofthesamplebytheproceduredescribedinthisInternationalStandard,dividedbythemassofthetestportionNOTEThecrudefibrecontentisexpressedingramsperkilogram.Itmayalsobeexpressedasamassfractioninpercent.4PrincipleThetestportionistreatedwithboilingdilutesulfuricacid.Theresidueisseparatedbyfiltration,washedandthentreatedwithboilingpotassiumhydroxidesolution.Theresidueisseparatedbyfiltration,washed,dried,weighedandthenashed.Thelossinmassresultingfromashingcorrespondstothemassofcrudefibreinthetestportion.SIST EN ISO 6865:2001
ISO6865:2000(E)2©ISO2000–Allrightsreserved5ReagentsandmaterialsUseonlyreagentsofrecognizedanalyticalgrade.5.1Water,complyingwithatleastgrade3inaccordancewithISO3696.5.2Hydrochloricacid,c(HCl)=0,5mol/l.5.3Sulfuricacid,c(H2SO4)=(0,130,005)mol/l.5.4Potassiumhydroxidesolution,c(KOH)=(0,230,005)mol/l.5.5Acetone.5.6Filteraid:seasand,orCelite®1)545,ormaterialofanequivalentquality.Beforeuse,treatseasandwithboilinghydrochloricacid[c(HCl)=4mol/l],washwithwateruntilfreefromacidandheatatatemperatureof(50025)°Cforatleast1h.Beforeuse,heatotherfilteraidsatatemperatureof(50025)°Cforatleast4h.5.7Antifoamagent,forinstancen-octanol.5.8Lightpetroleum,boilingrange40°Cto60°C.6ApparatusUsuallaboratoryapparatusand,inparticular,thefollowing.6.1Grindingdevice,capableofgrindingthesamplesothatitpassescompletelythroughasievewith1mmapertures.6.2Analyticalbalance,withaccuracyofatleast0,1mg.6.3Filtercrucibles,ofquartz,porcelainorhardglass,withfusedsinteredfilterplatewithaporesizeof40mto100m(porositygradeP100accordingtoISO4793:1980[1]).Beforeusingforthefirsttime,carefullyandgraduallyheatanewfiltercrucibletoatemperaturenotexceeding525°Candleaveforafewminutesat(50025)°C.Stainless-steelcrucibleswithstainless-steelsieveplateswithanaperturesizeof90mofidenticalperformancecharacteristicsmaybeusedasanalternative.6.4Porcelainsieveplates.6.5Incinerationdishes.6.6Beakersorconicalflasks,of500mlcapacity,providedwithasuitablecoolingdevice,forexampleacondensororadish.1)Celite®isthetradenameofacommerciallyavailableproduct.ThisinformationisgivenfortheconvenienceofusersofthisInternationalStandardanddoesnotconstituteanendorsementbyISOofthisproduct.Equivalentproductsmaybeusediftheycanbeshowntoleadtothesameresults.SIST EN ISO 6865:2001
ISO6865:2000(E)©ISO2000–Allrightsreserved36.7Dryingoven,electricallyheatedandventilated,capableofbeingmaintainedatatemperatureof(1302)°C.6.8Desiccator,containingbluesilicagelasdesiccant,providedwithaperforatedplate,preferablyofaluminiumorstainlesssteel,ofthickness2mmto3mm.6.9Mufflefurnace,electricallyheated,providedwithaircirculationandtemperaturecontrolcapableofmaintainingthetemperaturearoundthecruciblestothenearest25°Cattemperaturesof475°Cto525°C.Thereadingofthepyrometerofthemufflefurnacecannotalwaysbetrusted:deviationsmayoccur.Therefore,thetemperatureinthemufflefurnaceshouldbecheckedregularly.Dependingonthesizeandtypeofthemufflefurnace,temperaturesinthefurnacemaydifferfromoneplacetoanotherplace.Whentheovendoorisclosed,adequateairsupplyshouldbeguaranteed.Thevolumeflowrateofairshouldnotbesogreatthatmaterialiscarriedawayfromthecrucibles.6.10Cold-extractiondevice,providedwithasupportforthefiltercrucible(6.3);adischargepipewithataptothevacuumandliquidoutlet;andconnectingringsforconnectingthefiltercrucible(6.3).6.11Heatingdevice(formanualmethod),providedwithasuitablecoolingdevicecapableofmaintainingthevolumeconstantduringboiling.6.12Heatingdevice(forthesemi-automaticmethod)foracidandalkalinedigestion,providedwithasupportforthefiltercrucible(6.3);adischargepipewithataptothevacuumandliquidoutlet;aboilingcylinderofatleast270mlcapacity,witharefluxcondenser;connectingringsforconnectingtheheatingdevicetothefiltercrucible(6.3)andtheboilingcylinder;andoptionally,provisionforcompressedair.Beforeuse,preheattheapparatuswithboilingwaterfor5min.7SamplingSamplingisnotpartofthemethodspecifiedinthisInternationalStandard.ArecommendedsamplingmethodisgiveninISO6497[6].Itisimportantthatthelaboratoryreceiveasamplewhichistrulyrepresentativeandhasnotbeendamagedorchangedduringtransportorstorage.8PreparationoftestsamplePreparethetestsampleinaccordancewithISO6498.Usingthegrindingdevice(6.1),grindtheair-drylaboratorysamplesothatitpassescompletelythroughasievewith1mmapertures.Mixthoroughly.SIST EN ISO 6865:2001
ISO6865:2000(E)4©ISO2000–AllrightsreservedForthemanualmethod,proceedinaccordancewithclause9.Forthesemi-automaticmethod,proceedinaccordancewithclause10.9Procedureformanualmethod9.1TestportionWeighabout1gofthepreparedtestsample(clause8)tothenearest0,1mg(m1).Ifthefatcontentofthesampleexceeds100g/kg,orifthesamplecontainsfatswhichcannotbeextracteddirectlywithlightpetroleum(5.8),transferthesampletoacrucible(6.3)andproceedinaccordancewith9.2.Ifthefatcontentofthesampledoesnotexceed100g/kg,transferthesampletoabeaker(6.6)andproceedinaccordancewith9.3ifthecarbonatecontent,expressedascalciumcarbonate,exceeds50g/kg.Ifthisisnotthecase,proceedinaccordancewith9.4.9.2PreliminarydefattingInthecold-extractiondevice(6.10),defatthesamplethreetimesundervacuumwith30mloflightpetroleum(5.8)eachtime.Drytheresiduebysuctionaftereachwashing.Transfertheresiduetoabeaker(6.6).Ifthecarbonatecontent,expressedascalciumcarbonate,exceeds50g/kg,proceedinaccordancewith9.3.Ifnot,proceedinaccordancewith9.4.9.3RemovalofcarbonatePour100mlofhydrochloricacid(5.2)overthesample.Stircontinuouslyfor5min.Carefullypourthemixtureintoafiltercrucible,thebottomofwhichiscoveredwithathinlayeroffilteraid(5.6).Decanttwicewith100mlofwatereachtime.Takecaresothataslittlematerialaspossibleendsuponthefilter.Transferthecontentsofthecrucibletotheoriginalbeakerandproceedinaccordancewith9.4.9.4AciddigestionPour150mlofsulfuricacid(5.3)overthesample.Bringtotheboilasquicklyaspossibleandcontinuesteadyboilingfor(301)min.Atthebeginningofboiling,swirlafewtimes.Iffoamingoccurs,addafewdropsofantifoamagent(5.7).Duringboiling,maintainaconstantvolumebyusingasuitablecoolingdevice(see6.6and6.11).9.5FirstfiltrationInthefiltercrucible(6.3)applyalayeroffilteraid
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