SIST EN 12469-5:2026

SLOVENSKI STANDARD
01-januar-2026
Biološke varnostne omare - 5. del: Vgradnja, prevzemni postopki in rutinsko
preskušanje
Biological safety cabinets - Part 5: Installation, commissioning and routine testing
Biologische Sicherheitswerkbänke - Teil 5: Aufstellung, Inbetriebnahme und Wartung
Postes de sécurité biologique - Partie 5: Installation, mise en service et essais de routine
Ta slovenski standard je istoveten z: EN 12469-5:2025
ICS:
07.080 Biologija. Botanika. Zoologija Biology. Botany. Zoology
07.100.01 Mikrobiologija na splošno Microbiology in general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 12469-5
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2025
EUROPÄISCHE NORM
ICS 07.100.01
English Version
Biological safety cabinets - Part 5: Installation,
commissioning and routine testing
Postes de sécurité microbiologique - Partie 5 : Biologische Sicherheitswerkbänke - Teil 5: Aufstellung,
Installation, mise en service et essais de routine Inbetriebnahme und Wartung
This European Standard was approved by CEN on 5 October 2025.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 12469-5:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Installation of BSC . 6
4.1 General. 6
4.2 Information required for installation of BSC . 6
4.3 Recommendations for placement of BSC class I and class II . 7
4.3.1 General. 7
4.3.2 Impact of extract-, make-up and supply air systems . 7
4.3.3 Impact of other equipment . 7
4.3.4 Space requirements . 7
4.3.5 Recommendations for the number of persons working at a BSC at the same time . 11
5 Commissioning of BSC . 11
5.1 General. 11
5.2 General inspections. 12
6 Routine testing and maintenance of BSC . 12
6.1 General. 12
6.2 Decontamination and disinfection . 12
6.3 Biological safety cabinets temporarily out of commission . 12
7 Test reports . 13
7.1 Reports for commissioning . 13
7.2 Reports for routine testing . 13
7.3 Report for maintenance . 14
7.4 Deviation . 14
Annex A (informative) Training . 15
Bibliography . 16

European foreword
This document (EN 12469-5:2025) has been prepared by Technical Committee CEN/TC 332 “Laboratory
Equipment”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2026, and conflicting national standards shall be
withdrawn at the latest by May 2026.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
and
This document, together with EN 12469-1:2025, EN 12469-2:2025, prEN 12469-3:—
prEN 12469-4:— , will partially supersede EN 12469:2000.
— the structure has been changed to emphasize different classes of biological safety cabinets (BSC);
— the text of the entire document has been revised and references have been updated.
EN 12469:2025 consists of the following parts, under the general title Biological safety cabinets:
— Part 1: Classes, terminology and basic requirements
— Part 2: BSC class II
— Part 3: BSC class III
— Part 4: BSC class I
— Part 5: Installation, commissioning and routine testing
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Under preparation.
Introduction
Biological safety cabinets (BSC) are designed to protect the operator and the environment against the
risks associated with the handling of biological agents. Depending on the classes, a BSC can additionally
protect the product.
Each BSC class has its own design and performance criteria. The choice of a BSC class depends on the type
of protection required and the assessment of the risk to be controlled.
EN 12469 describes the BSC classes, their design, correct usage, and testing principles.
This document is a product standard. Occupational health and safety assessments methods are not
included.
1 Scope
This document gives requirements and recommendations for installation, commissioning and routine
testing of BSC.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 12469-1, Biological safety cabinets - Part 1: Classes and basic requirements
CEN/TS 17441, Laboratory installations - Ventilation systems in laboratories
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 12469-1 and the following apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp/
— IEC Electropedia: available at https://www.electropedia.org/
3.1
commissioning
process of ensuring that the BSC is installed and tested according to this document
3.2
corrective maintenance
maintenance carried out after fault recognition and intended to restore an item into a state in which it
can perform a required function
[SOURCE: EN 13306:2017, 7.9]
3.3
hard duct
connection between two air handling devices that does not have any open sections
3.4
installation
physical placement including connection of mechanical, electrical, and communication services within a
building
3.5
maintenance
combination of all technical, administrative and managerial actions during the life cycle of an item
intended to retain it in, or restore it to, a state in which it can perform the required function
Note 1 to entry: Technical maintenance actions include observation and analyses of the item state (e.g. inspection,
monitoring, testing, diagnosis, prognosis, etc.) and active maintenance actions (e.g. repair, refurbishment).
[SOURCE: EN 13306:2017, 2.1, modified, Notes 2 and 3 to entry removed]
3.6
preventive maintenance
maintenance carried out intended to assess and/or to mitigate degradation and reduce the probability of
failure of an item
[SOURCE: EN 13306:2017, 7.1]
3.7
routine testing
test protocols carried out at predetermined intervals, intended to confirm that the BSC is operating within
manufacturers specifications
3.8
thimble
duct connection device that provides an air break between two air handling devices that prevents adverse
pressure situations when either of the devices fail
4 Installation of BSC
4.1 General
This clause gives recommendations and guidance on the installation of BSC, including placement and
connection to and interfacing with building ventilation and utility services.
4.2 Information req
...