EN ISO 5398-4:2019
(Main)Leather - Chemical determination of chromic oxide content - Part 4: Quantification by inductively coupled plasma (ICP) (ISO 5398-4:2018)
General Information
- Abstract
This document describes a method for the determination of chromium in aqueous solution obtained from leather. This is an analysis for total chromium in leather; it is not compound specific or specific to its oxidation state.
This method describes the determination of chromium by inductively coupled plasma (ICP) and is applicable to leathers which are expected to have chromic oxide contents in excess of 1 mg/kg. Two techniques for the preparation of the solution to be analysed are included. In the event of dispute, the wet oxidation technique is intended to be used.
- Status
- Published
- Publication Date
- 05-Feb-2019
- Withdrawal Date
- 30-Aug-2019
- Technical Committee
- CEN/TC 289 - Leather
- Drafting Committee
- CEN/TC 289/WG 1 - Chemical test methods
- Current Stage
- 6060 - Definitive text made available (DAV) - Publishing
- Start Date
- 06-Feb-2019
- Completion Date
- 06-Feb-2019
Overview
EN ISO 5398-4:2019 - Leather - Chemical determination of chromic oxide content - Part 4: Quantification by inductively coupled plasma (ICP) - specifies a laboratory method for measuring total chromium in leather extracts using inductively coupled plasma techniques (ICP-OES or ICP-MS). The method reports results as chromic oxide (Cr2O3) in mg/kg (dry matter) and is applicable to leathers expected to contain chromic oxide in excess of 1 mg/kg. This standard is the CEN adoption of ISO 5398-4:2018 and supersedes the 2007 edition.
Key topics and technical requirements
- Scope: Determination of total chromium in aqueous solutions obtained from leather. Not compound- or oxidation-state-specific.
- Preparation techniques: Two preparation methods are included:
- Wet oxidation (recommended in case of dispute). Warning: add nitric acid first to avoid hazardous reactions with perchloric acid. Typical analytical sample mass guidance: 2 g of prepared leather (perform duplicate determinations if possible).
- Microwave digestion (or other validated digestion methods) using ultrapure acids.
- Analytical techniques: Measurement by ICP-OES (per ISO 11885) or ICP-MS (per ISO 17294-2). Annex B provides guidance on suitable ICP equipment.
- Reagents & quality: Analytical-grade reagents (nitric, sulfuric/perchloric mix), ultrapure acids for microwave digestion, and grade 3 water per ISO 3696. Commercial chromium standards (e.g., 1 000 mg/L) are used for calibration.
- Apparatus: ICP instrument, filtration (glass-fibre or membrane), anti-bumping granules (for wet oxidation), volumetric flasks, etc.
- Reporting: Chromium content is expressed as chromic oxide (mg/kg) based on dry matter; follow sample preparation and sampling rules (ISO 2418, ISO 4044).
Practical applications and users
- Who uses it: Leather testing laboratories, tanneries, quality control departments, material compliance teams, research institutes and forensic labs analyzing leather contaminants.
- Why it’s used: Provides a precise, reproducible method for quantifying chromium levels in leather for product quality control, process monitoring, comparative testing, and laboratory accreditation.
- Benefits: ICP methods deliver higher precision and sensitivity than simpler colorimetric or titrimetric techniques, and are suitable where accurate total chromium measurement above ~1 mg/kg is required.
Related standards
- ISO 2418 - Sampling location for leather testing
- ISO 4044 - Preparation of chemical test samples (leather)
- ISO 3696 - Water for analytical laboratory use
- ISO 11885 - ICP-OES for selected elements
- ISO 17294-2 - ICP-MS application for selected elements
- ISO 4684 - Determination of volatile matter (informative for dry-mass reporting)
Keywords: EN ISO 5398-4:2019, leather chromic oxide, chromium determination, ICP-OES, ICP-MS, wet oxidation, microwave digestion, leather testing.
Relations
- Effective Date
- 10-May-2017
- Effective Date
- 25-Aug-2026
- Effective Date
- 25-Aug-2026
- Effective Date
- 25-Aug-2026
- Effective Date
- 09-Feb-2026
- Effective Date
- 09-Feb-2026
- Refers
ISO 2418:2017 - Leather — Chemical, physical and mechanical and fastness tests — Sampling location - Effective Date
- 09-Feb-2026
- Effective Date
- 28-Jan-2026
- Effective Date
- 28-Jan-2026
- Referred By
EN 16419:2014 - Leather - Chamois leather for cleaning purposes - Classification and requirements - Effective Date
- 28-Jan-2026
- Effective Date
- 12-Feb-2026
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Frequently Asked Questions
EN ISO 5398-4:2019 is a standard published by the European Committee for Standardization (CEN). Its full title is "Leather - Chemical determination of chromic oxide content - Part 4: Quantification by inductively coupled plasma (ICP) (ISO 5398-4:2018)". This standard covers: This document describes a method for the determination of chromium in aqueous solution obtained from leather. This is an analysis for total chromium in leather; it is not compound specific or specific to its oxidation state. This method describes the determination of chromium by inductively coupled plasma (ICP) and is applicable to leathers which are expected to have chromic oxide contents in excess of 1 mg/kg. Two techniques for the preparation of the solution to be analysed are included. In the event of dispute, the wet oxidation technique is intended to be used.
This document describes a method for the determination of chromium in aqueous solution obtained from leather. This is an analysis for total chromium in leather; it is not compound specific or specific to its oxidation state. This method describes the determination of chromium by inductively coupled plasma (ICP) and is applicable to leathers which are expected to have chromic oxide contents in excess of 1 mg/kg. Two techniques for the preparation of the solution to be analysed are included. In the event of dispute, the wet oxidation technique is intended to be used.
EN ISO 5398-4:2019 is classified under the following ICS (International Classification for Standards) categories: 59.140.30 - Leather and furs; 71.040.50 - Physicochemical methods of analysis. The ICS classification helps identify the subject area and facilitates finding related standards.
EN ISO 5398-4:2019 has the following relationships with other standards: It is inter standard links to EN ISO 5398-4:2007, ISO 17294-2:2016, ISO 11885:2007, ISO 4684:2005, ISO 4044:2017, ISO 3696:1987, ISO 2418:2017, EN 2240-065:2011, EN 1457-1:2012, EN 16419:2014, ISO 5398-4:2018. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
EN ISO 5398-4:2019 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
01-april-2019
1DGRPHãþD
SIST EN ISO 5398-4:2009
8VQMH.HPLMVNRGRORþHYDQMHNURPRYHJDRNVLGDGHO.YDQWLWDWLYQR
GRORþHYDQMH]LQGXNWLYQRVNORSOMHQRSOD]PR,&3,62
Leather - Chemical determination of chromic oxide content - Part 4: Quantification by
inductively coupled plasma (ICP) (ISO 5398-4:2018)
Leder - Chemische Bestimmung des Chromoxidgehalts - Teil 4: Quantitative
Bestimmung durch induktiv gekoppeltes Plasma (ICP) (ISO 5398-4:2018)
Cuir - Dosage chimique de l'oxyde de chrome - Partie 4: Quantification par plasma à
couplage inductif (ICP) (ISO 5398-4:2018)
Ta slovenski standard je istoveten z: EN ISO 5398-4:2019
ICS:
59.140.30 Usnje in krzno Leather and furs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 5398-4
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2019
EUROPÄISCHE NORM
ICS 59.140.30 Supersedes EN ISO 5398-4:2007
English Version
Leather - Chemical determination of chromic oxide content
- Part 4: Quantification by inductively coupled plasma
(ICP) (ISO 5398-4:2018)
Cuir - Dosage chimique de l'oxyde de chrome - Partie 4: Leder - Chemische Bestimmung des Chromoxidgehalts
Quantification par plasma à couplage inductif (ICP) - Teil 4: Quantitative Bestimmung durch induktiv
(ISO 5398-4:2018) gekoppeltes Plasma (ICP) (ISO 5398-4:2018)
This European Standard was approved by CEN on 16 December 2018.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 5398-4:2019 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 5398-4:2019) has been prepared by Technical Committee ISO/IULTCS
"International Union of Leather Technologists and Chemists Societies" in collaboration with Technical
Committee CEN/TC 289 “Leather” the secretariat of which is held by UNI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2019, and conflicting national standards shall
be withdrawn at the latest by August 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 5398-4:2007.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 5398-4:2018 has been approved by CEN as EN ISO 5398-4:2019 without any
modification.
INTERNATIONAL ISO
STANDARD 5398-4
IULTCS/IUC 8-4
Second edition
2018-12
Leather — Chemical determination of
chromic oxide content —
Part 4:
Quantification by inductively coupled
plasma (ICP)
Cuir — Dosage chimique de l'oxyde de chrome —
Partie 4: Quantification par plasma à couplage inductif (ICP)
Reference numbers
ISO 5398-4:2018(E)
IULTCS/IUC 8-4:2018(E)
©
ISO 2018
ISO 5398-4:2018(E)
IULTCS/IUC 8-4:2018(E)
© ISO 2018
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
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Phone: +41 22 749 01 11
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Email: copyright@iso.org
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Published in Switzerland
ii © ISO 2018 – All rights reserved
ISO 5398-4:2018(E)
IULTCS/IUC 8-4:2018(E)
Contents Page
Foreword .iv
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Sampling and sample preparation . 2
6 Reagents . 2
7 Apparatus . 2
8 Methods . 2
8.1 Preparation of analytical solution . 2
8.1.1 Wet oxidation method . 2
8.1.2 Microwave digestion or other method of digestion . 3
8.2 Measurement of the aqueous solution . 3
8.2.1 General. 3
8.2.2 Preparation of calibration graph . 3
8.2.3 Analysis of the test solution . 3
9 Calculation and expression of results . 4
10 Test report . 4
Annex A (informative) Determination of water and other volatile matter . 5
Annex B (informative) Suitable ICP equipment . 6
ISO 5398-4:2018(E)
IULTCS/IUC 8-4:2018(E)
Foreword
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This document was prepared by the Chemical Tests Commission of the International Union of Leather
Technologists and Chemists Societies (IUC Commission, IULTCS) in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 289, Leather, the secretariat of
which is held by UNI, in accordance with the Agreement on technic
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