EN 16294:2012
(Main)Petroleum products and fat and oil derivatives - Determination of phosphorus content in fatty acid methyl esters (FAME) - Optical emission spectral analysis with inductively coupled plasma (ICP OES)
General Information
- Abstract
This European Standard specifies an inductively coupled plasma optical emission spectrometry (ICP OES) method for the determination of phosphorus content of Fatty Acid Methyl Esters (FAME) in the range of 2,5 mg/kg to 8,0 mg/kg.
WARNING - The use of this standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
- Status
- Published
- Publication Date
- 27-Nov-2012
- Withdrawal Date
- 30-May-2013
- Technical Committee
- CEN/TC 19 - Petroleum products, lubricants and related products
- Drafting Committee
- CEN/TC 19/WG 27 - Elemental analysis of petroleum and related products
- Current Stage
- 9093 - Decision to confirm - Review Enquiry
- Start Date
- 11-Jan-2024
- Completion Date
- 23-Sep-2026
Overview
EN 16294:2012 (CEN) defines an analytical method for the determination of phosphorus content in Fatty Acid Methyl Esters (FAME) using inductively coupled plasma optical emission spectrometry (ICP OES). The standard covers direct optical emission spectral analysis of FAME samples and is intended for phosphorus concentrations in the range 2.5 mg/kg to 8.0 mg/kg. A general safety warning highlights that hazardous materials and equipment may be involved and that users must adopt appropriate safety practices.
Key topics and technical requirements
- Scope and target analyte: Phosphorus in FAME (2.5–8.0 mg/kg).
- Analytical technique: ICP OES (optical emission spectrometry with inductively coupled plasma). Radial ICP-OES is recommended to minimize carbon-related matrix effects.
- Sample preparation: Weigh FAME, dilute with kerosene (boiling range 175–250 °C) and use oil-based stock and intermediate standards. Use plastic bottles (HDPE, FEP, PFA) to avoid contamination.
- Internal standard: Use element standards such as cobalt, scandium or yttrium to correct viscosity/matrix effects.
- Calibration: Two procedures described (A and B) that use intensity ratios or corrected intensities; calibration uses multiple standards and linear regression. Recommended P emission lines include 177.499 nm, 178.287 nm, 213.618 nm, 214.914 nm.
- Quality control: Regular calibration checks with QC samples; if QC differs by more than 20% from reference, recalibrate.
- Equipment and reagents: High-purity argon (≥ 99.996%), appropriate nebuliser and pump tubing for organic solvents, analytical balance (0.1 mg), volumetric flasks and solvent-cleaning protocols to avoid phosphorus contamination.
- Performance data: Contains precision statements (repeatability and reproducibility) and reporting requirements.
Applications and users
- Biodiesel producers and quality control labs assessing phosphorus in FAME to ensure fuel quality and protect engine and exhaust after‑treatment systems.
- Fuel testing and certification laboratories performing routine compositional analysis and regulatory compliance testing.
- Research laboratories studying feedstock impacts and refining processes that affect phosphorus levels.
- Refineries and blending facilities monitoring additives and contaminants during production and blending.
Practical benefits: accurate low-level phosphorus measurement, matrix correction via internal standards, and standardized calibration/QA procedures for consistent results across laboratories.
Related standards
- EN ISO 1042 - Laboratory glassware: one-mark volumetric flasks
- EN ISO 3170 - Petroleum liquids - Manual sampling
- EN ISO 3171 - Petroleum liquids - Automatic pipeline sampling
Keywords: EN 16294:2012, FAME phosphorus analysis, ICP OES, inductively coupled plasma, phosphorus in biodiesel, calibration, internal standard, fuel testing, CEN.
Relations
- Effective Date
- 28-Jan-2026
- Effective Date
- 28-Jan-2026
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Frequently Asked Questions
EN 16294:2012 is a standard published by the European Committee for Standardization (CEN). Its full title is "Petroleum products and fat and oil derivatives - Determination of phosphorus content in fatty acid methyl esters (FAME) - Optical emission spectral analysis with inductively coupled plasma (ICP OES)". This standard covers: This European Standard specifies an inductively coupled plasma optical emission spectrometry (ICP OES) method for the determination of phosphorus content of Fatty Acid Methyl Esters (FAME) in the range of 2,5 mg/kg to 8,0 mg/kg. WARNING - The use of this standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
This European Standard specifies an inductively coupled plasma optical emission spectrometry (ICP OES) method for the determination of phosphorus content of Fatty Acid Methyl Esters (FAME) in the range of 2,5 mg/kg to 8,0 mg/kg. WARNING - The use of this standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
EN 16294:2012 is classified under the following ICS (International Classification for Standards) categories: 75.160.20 - Liquid fuels; 75.160.40 - Biofuels. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 16294:2012 has the following relationships with other standards: It is inter standard links to EN 14214:2012+A2:2019, EN 14214:2012+A1:2014. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
EN 16294:2012 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.NWLYQRMineralölprodukte und Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Bestimmung des Gehalts an Phosphor in Fettsäuremethylestern (FAME) - Direkte Bestimmung durch optische Emissionsspektralanalyse mit induktiv gekoppeltem Plasma (ICP OES)Produits pétroliers et produits dérivés des corps gras - Détermination de la teneur en phosphore des esters méthyliques d'acides gras (EMAG) - Méthode par spectrométrie d'émission atomique à couplage inductif par plasma (ICP OES)Petroleum products and fat and oil derivatives - Determination of phosphorus content in fatty acid methyl esters (FAME) - Optical emission spectral analysis with inductively coupled plasma (ICP OES)75.160.20Liquid fuelsICS:Ta slovenski standard je istoveten z:EN 16294:2012SIST EN 16294:2013en,de01-februar-2013SIST EN 16294:2013SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16294
November 2012 ICS 75.160.20 English Version
Petroleum products and fat and oil derivatives - Determination of phosphorus content in fatty acid methyl esters (FAME) - Optical emission spectral analysis with inductively coupled plasma (ICP OES)
Produits pétroliers et produits dérivés des corps gras - Détermination de la teneur en phosphore des esters méthyliques d'acides gras (EMAG) - Méthode par spectrométrie d'émission atomique par plasma
à couplage inductif (ICP OES)
Mineralölprodukte und Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Bestimmung des Gehalts an Phosphor in Fettsäuremethylestern (FAME) - Direkte Bestimmung durch optische Emissionsspektralanalyse mit induktiv gekoppeltem Plasma (ICP OES) This European Standard was approved by CEN on 13 October 2012.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16294:2012: ESIST EN 16294:2013
4.4 Phosphorus intermediate solution (25 mg/kg).
Weigh 0,50 g ± 0,01 g of phosphorus standard solution (4.3) into a 50 ml bottle (5.2). Add kerosene (4.2) to 20,00 g ± 0,01 g. Each mass shall be weighed to the nearest 0,1 mg. SIST EN 16294:2013
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