EN 17163:2019

SLOVENSKI STANDARD
01-april-2019
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Pulp, paper and board - Determination of primary aromatic amines (PAA) in a water
extract by a LC-MS method
Papier, Pappe und Faserstoff - Bestimmung von primären aromatischen Aminen in
Wasserextrakten mittels LC-MS
Pâtes, papier et carton - Détermination des amines aromatiques primaires (AAP) dans
un extrait aqueux par méthode LC-MS
Ta slovenski standard je istoveten z: EN 17163:2019
ICS:
85.040 Vlaknine Pulps
85.060 Papir, karton in lepenka Paper and board
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 17163
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2019
EUROPÄISCHE NORM
ICS 85.060
English Version
Pulp, paper and board - Determination of primary
aromatic amines (PAA) in a water extract by a LC-MS
method
Pâtes, papier et carton - Détermination des amines Papier, Pappe und Faserstoff - Bestimmung von
aromatiques primaires (AAP) dans un extrait aqueux primären aromatischen Aminen in Wasserextrakten
par méthode LC-MS mittels LC-MS
This European Standard was approved by CEN on 26 November 2018.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17163:2019 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
4.1 General . 5
4.2 Interferences . 6
5 Apparatus . 6
6 Reagents . 6
7 Sample preparation . 10
8 HPLC conditions . 10
8.1 General . 10
8.2 System example 1 . 11
8.3 System example 2 . 11
8.4 Example of MS conditions . 12
8.5 HPLC calibration . 12
8.6 Quality control . 13
8.7 Confirmation of Identity . 13
9 Calculation of the PAA concentrations in the Sample Extracts . 13
10 Test report . 13
Annex A (informative) Precision data . 14
Bibliography . 16

European foreword
This document (EN 17163:2019) has been prepared by Technical Committee CEN/TC 172 “Pulp, paper
and board”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2019, and conflicting national standards shall
be withdrawn at the latest by August 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Introduction
Primary aromatic amines (PAAs) can occur in paper and board materials as residual basic chemicals for
the production of azodyes and azo pigments. Furthermore, hydrolysis products of isocyanates
especially from paper laminates may be a possible source.
A draft of a European Standard EN 13130-XX concerning the “Determination of Primary Aromatic
Amines (PAAs) in Food Simulants” has been prepared by the standardization committee
CEN/TC 194/SC1/TG9.
When preparing this standard it has been assessed by the laboratories participating that the
photometric screening method described in part A of the draft of standard EN 13130-XX is not
applicable for the examination of water extracts of paper and board materials due to interferences of
extractable paper components which caused irreproducible test results.
Due to the advantages of the MS/MS-detection regarding selectivity and sensitivity the determination of
primary aromatic amines by means of LC-MS/MS was found to be capable for water extracts of paper
and board materials when preparing this standard.
1 Scope
This document describes two representative methods for the determination of the extractable amount
of specific primary aromatic amines (PAA) in a water extract of paper, board and pulp samples by
means of HPLC with MS/MS detection which basically differ concerning the choice of the mobile and
stationary phases. Deviating from this standard further methods may be applicable if it can be shown
that comparable results can be achieved. A validation should be carried out by each laboratory.
It is applicable for the determination of the 22 primary aromatic amines (PAA) mentioned in the annex
of Directive 2002/61/EC of 19th July 2002 amending Council Directive 76/769/EEC relating to
restrictions on the market and use of certain dangerous substances and preparations (azocolourants)
which are classified as carcinogenic categories 1A and 1B according to the CLP regulation and aniline.
The method described by this standard should be also applicable for the determination of further
primary aromatic amines (PAA). A validation for every further analyte has to be done by the laboratory
using this method.
The extractable amount of a primary aromatic amine (PAA) is expressed in mg PAA per litre water
extract. This method is suitable for the determination of PAA with a working range of about
0,001 mg/l – 0,020 mg/l water extract.
Deviating from this standard further methods may be applicable if it can be shown that comparable
results can be achieved. A validation should be carried out by each laboratory.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 645, Paper and board intended to come into contact with foodstuffs — Preparation of a cold water
extract
EN 647, Paper and board intended to come into contact with foodstuffs — Preparation of a hot water
extract
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
• IEC Electropedia: available at http://www.ele
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